expression and secretion of human granulocyte macrophage-colony stimulating factor using escherichia coli enterotoxin i signal sequence
Authors
abstract
with the aim of the secretion of human granulocyte macrophage-colony stimulating factor (hgm-csf) in escherichia coli, hgm-csf cdna was fused in-frame next to the signal sequence of st toxin (st-i) of exteroxigenic e. coli, containing 53 or 19 amino acids of signal peptide. the fused stsig::hgm-csf coding fragments were inserted into a t7-based expression plasmid. the recombinant plasmids were subjected to expression analysis of hgm-csf in the bl21(de3) strain of e. coli under iptg induction. based on the results obtained from the protein pattern of the recombinant e. coli under inducing condition, accumulation of the expressed target protein was detected in both cytoplasmic and periplasmic space
similar resources
Expression and Secretion of Human Granulocyte Macrophage-Colony Stimulating Factor Using Escherichia coli Enterotoxin I Signal Sequence
With the aim of the secretion of human granulocyte macrophage-colony stimulating factor (hGM-CSF) in Escherichia coli, hGM-CSF cDNA was fused in-frame next to the signal sequence of ST toxin (ST-I) of exteroxigenic E. coli, containing 53 or 19 amino acids of signal peptide. The fused STsig::hGM-CSF coding fragments were inserted into a T7-based expression plasmid. The recombinant plasmids were ...
full textThe Expression of Human Granulocyte Macrophage Colony Stimulating Factor by Heat-Induction in Escherichia coli
A self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pL promoter, was constructed. The designed 5024 base pair expression plasmid contained a heat sensitive repressor cI857 coding gene to regulate the function of ?pL promoter under heat shock induction. Using the constructed vector,...
full textSite-Directed Mutagenesis in Human Granulocyte-colony Stimulating Factor, Cloning and Expression in Escherichia coli
Human granulocyte colony stimulating factor (hG-CSF) induces proliferation and differentiation of granulocyte progenitor cells. This glycoprotein is currently being used for treatment of neutropenia, in patients who have undergone bone marrow transplantation. So far, different researchers have tried to enhance hG-CSF biological activity and stability. In this study, Polymerase Chain Reaction (P...
full textthe expression of human granulocyte macrophage colony stimulating factor by heat-induction in escherichia coli
a self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pl promoter, was constructed. the designed 5024 base pair expression plasmid contained a heat sensitive repressor ci857 coding gene to regulate the function of ?pl promoter under heat shock induction. using the constructed vector,...
full textEfficient Process Development of Recombinant Human Granulocyte Colony-Stimulating Factor (rh-GCSF) Production in Escherichia coli
Background: The protein hormone granulocyte colony-stimulating factor (GCSF) stimulates the production of white blood cells and plays an important role in medical treatment of cancer patients. Methods: An efficient process was developed for heterologous expression of human GCSF in E. coli BL21 (DE3). The feeding rate was adjusted to achieve the maximum attainable specific growth rate under crit...
full textMy Resources
Save resource for easier access later
Journal title:
journal of sciences islamic republic of iranجلد ۱۶، شماره ۴، صفحات ۰-۰
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023